6LT5
Lysozyme protected by alginate gel
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AichiSR BEAMLINE BL2S1 |
| Synchrotron site | AichiSR |
| Beamline | BL2S1 |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2019-12-11 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.12 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.097, 79.097, 36.962 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.840 - 1.320 |
| R-factor | 0.1704 |
| Rwork | 0.170 |
| R-free | 0.17960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dpx |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.780 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.370 | 1.320 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.053 | 0.562 |
| Number of reflections | 29447 | 1401 |
| <I/σ(I)> | 20.5 | 2 |
| Completeness [%] | 99.8 | 96.7 |
| Redundancy | 6.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | COUNTER-DIFFUSION | 4.5 | 277 | 50mM sodium acetate, 1.5M NaCl, pH 4.5 |
