6LSV

Crystal structure of JOX2 in complex with 2OG, Fe, and JA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9793
Spacegroup name	C 1 2 1
Unit cell lengths	153.476, 74.416, 75.488
Unit cell angles	90.00, 110.04, 90.00

Refinement procedure

Resolution	26.334 - 2.651
R-factor	0.197
Rwork	0.195
R-free	0.23400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gp4
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX (1.14rc2_3191: ???)
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.690
High resolution limit [Å]	2.640	2.640
Rmerge	0.131	0.479
Rpim	0.054	0.193
Number of reflections	23124	2225
<I/σ(I)>	16.1	4.9
Completeness [%]	99.0	96.49
Redundancy	6.8	7
CC(1/2)	0.988	0.934

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1M HEPES, pH 7.5, 1.26M Ammonium Sulfate