6LST
Crystal straucture of Uso1-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-05-08 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 114.368, 114.368, 193.443 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.220 - 2.940 |
| R-factor | 0.1948 |
| Rwork | 0.192 |
| R-free | 0.25160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2w3c |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.972 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.990 |
| High resolution limit [Å] | 2.940 | 7.970 | 2.940 |
| Rmerge | 0.088 | 0.051 | 0.896 |
| Rmeas | 0.094 | 0.055 | 0.948 |
| Rpim | 0.031 | 0.019 | 0.304 |
| Total number of observations | 282039 | ||
| Number of reflections | 31647 | 1710 | 1518 |
| <I/σ(I)> | 13.7 | ||
| Completeness [%] | 98.7 | 96.9 | 97.9 |
| Redundancy | 8.9 | 8.8 | 8.8 |
| CC(1/2) | 0.997 | 0.890 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M Tris-HCl pH 7.0, 0.2 M MgCl2, 2.5 M NaCl |
