6LSP
Crystal structure of a dimeric Piptidyl t-RNA hydrolase from Acinetobacter baumannii at 1.50 A resolution reveals an inhibited form.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | RRCAT INDUS-2 BEAMLINE PX-BL21 |
Synchrotron site | RRCAT INDUS-2 |
Beamline | PX-BL21 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-12-04 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 34.580, 98.540, 123.670 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.300 - 1.500 |
R-factor | 0.1701 |
Rwork | 0.170 |
R-free | 0.19680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5yn4 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.821 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.560 | 1.530 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.100 | 0.530 |
Rmeas | 0.108 | 0.580 |
Rpim | 0.042 | 0.220 |
Number of reflections | 68761 | 4972 |
<I/σ(I)> | 11.2 | 3.4 |
Completeness [%] | 100.0 | 99.92 |
Redundancy | 6.5 | 6.4 |
CC(1/2) | 0.990 | 0.860 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 20% PEG 1500, 0.1M HEPES, pH 7.5, 22% Glycerol |