6LSO
Crystal structure of a dimeric inhibited of peptidyl tRNA hydrolase at 1.76A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | RRCAT INDUS-2 BEAMLINE PX-BL21 |
Synchrotron site | RRCAT INDUS-2 |
Beamline | PX-BL21 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-12-04 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 34.560, 98.330, 123.930 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.740 - 1.760 |
Rwork | 0.183 |
R-free | 0.21840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5yn4 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.617 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.740 | 1.800 |
High resolution limit [Å] | 1.760 | 1.760 |
Rmerge | 0.130 | 0.470 |
Rmeas | 0.140 | 0.510 |
Rpim | 0.058 | 0.200 |
Number of reflections | 42085 | 3123 |
<I/σ(I)> | 8.4 | 2.9 |
Completeness [%] | 98.0 | 95 |
Redundancy | 6.2 | 5.9 |
CC(1/2) | 0.991 | 0.840 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 100mM HEPES, 20% PEG 1500 |