6LSG
Crystal structure of the enterovirus 71 polymerase elongation complex (C0S6M form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-12-08 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 63.564, 77.220, 154.164 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.370 - 2.140 |
R-factor | 0.1917 |
Rwork | 0.190 |
R-free | 0.21660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5f8g |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (v1.14) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.200 |
High resolution limit [Å] | 2.120 | 4.570 | 2.120 |
Rmerge | 0.031 | 0.024 | 0.201 |
Rmeas | 0.034 | 0.026 | 0.227 |
Rpim | 0.014 | 0.010 | 0.104 |
Total number of observations | 262212 | ||
Number of reflections | 42624 | 4647 | 3000 |
<I/σ(I)> | 18.9 | ||
Completeness [%] | 96.9 | 99.6 | 69.4 |
Redundancy | 6.2 | 6 | 4.5 |
CC(1/2) | 0.999 | 0.977 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 289 | MES, PEG 5000 monomethyl ether, glycerol |