6LS6
Crystal Structure of YEATS domain of AF9 in complex with H3K9bz peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-12-07 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9792 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 90.258, 42.236, 90.253 |
Unit cell angles | 90.00, 96.64, 90.00 |
Refinement procedure
Resolution | 44.824 - 2.200 |
R-factor | 0.2301 |
Rwork | 0.227 |
R-free | 0.27180 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4tmp |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.14rc3_3206) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.240 |
High resolution limit [Å] | 2.196 | 5.970 | 2.200 |
Rmerge | 0.139 | 0.077 | 0.422 |
Rmeas | 0.159 | 0.088 | 0.492 |
Rpim | 0.075 | 0.040 | 0.249 |
Number of reflections | 16939 | 868 | 808 |
<I/σ(I)> | 5.7 | ||
Completeness [%] | 96.6 | 93.5 | 94 |
Redundancy | 4 | 4.5 | 3.3 |
CC(1/2) | 0.992 | 0.923 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | 0.1 M Bis-Tris, pH 5.5??200 mM lithium sulfate, 25% w/v PEG 3350. |