6LS1

Ribonuclease from Hericium erinaceus active and GMP binding form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-01
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.100
Spacegroup name	I 4 2 2
Unit cell lengths	97.760, 97.760, 68.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.793 - 1.580
R-factor	0.1509
Rwork	0.149
R-free	0.19020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5gy6
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.880	1.610
High resolution limit [Å]	1.580	1.580
Number of reflections	23162	23162
<I/σ(I)>	23.3
Completeness [%]	100.0
Redundancy	22.6
CC(1/2)	0.999	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.4M Magnesium sulfate, 20% PEG 3350