6LRV

Marsupenaeus japonicus ferritin mutant(T158H) pH9.0

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	273.15
Detector technology	PIXEL
Collection date	2019-01-14
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9785
Spacegroup name	P 2 3
Unit cell lengths	117.922, 117.922, 117.922
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.300
R-factor	0.1892
Rwork	0.188
R-free	0.21540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6a4u
RMSD bond length	0.019
RMSD bond angle	1.877
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	117.920	50.000	2.340
High resolution limit [Å]	2.300	6.240	2.300
Rmerge	0.106	0.041	0.283
Rmeas	0.109	0.042	0.290
Rpim	0.025	0.010	0.065
Number of reflections	24518	1322	1201
<I/σ(I)>	7.4
Completeness [%]	100.0	100	100
Redundancy	19.7	18.2	19.9
CC(1/2)		1.000	0.982

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	2500 mM NaCl, 100 mM KH2PO3 / KH2PO3 (pH 8.0)