6LRH
Crystal Structure of the Binary Complex of AgrE C264A mutant with L-arginine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 11C |
Synchrotron site | PAL/PLS |
Beamline | 11C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-10-29 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.979 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 186.157, 201.457, 98.916 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.458 - 2.705 |
R-factor | 0.195140735613 |
Rwork | 0.193 |
R-free | 0.24451 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6lrf |
RMSD bond length | 0.011 |
RMSD bond angle | 1.158 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (1.9_1692) |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Number of reflections | 50860 | 5040 |
<I/σ(I)> | 7.53 | |
Completeness [%] | 99.8 | |
Redundancy | 6.6 | |
CC(1/2) | 0.941 | 0.529 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 295.15 | sodium acetate, sodium citrate (pH5.5), PEG4000, glycerol |