6LQF

Crystal structure of Arabidopsis ARID5 ARID-PHD cassette in complex with H3K4me3 peptide and DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-20
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	56.173, 59.428, 92.958
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.035 - 1.500
R-factor	0.1759
Rwork	0.175
R-free	0.19160
Structure solution method	SAD
RMSD bond length	0.014
RMSD bond angle	1.612
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.530
High resolution limit [Å]	1.500	4.070	1.500
Rmerge	0.062	0.037	0.746
Rmeas	0.069	0.043	0.836
Rpim	0.030	0.021	0.369
Total number of observations	246245
Number of reflections	49942	2408	2473
<I/σ(I)>	9.5
Completeness [%]	98.2	87.2	97.9
Redundancy	4.9	3.6	4.7
CC(1/2)		0.997	0.779

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5	298	0.1 M sodium acetate, pH 5.0, 15% MPD