6LQF
Crystal structure of Arabidopsis ARID5 ARID-PHD cassette in complex with H3K4me3 peptide and DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-20 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.173, 59.428, 92.958 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.035 - 1.500 |
R-factor | 0.1759 |
Rwork | 0.175 |
R-free | 0.19160 |
Structure solution method | SAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.612 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.530 |
High resolution limit [Å] | 1.500 | 4.070 | 1.500 |
Rmerge | 0.062 | 0.037 | 0.746 |
Rmeas | 0.069 | 0.043 | 0.836 |
Rpim | 0.030 | 0.021 | 0.369 |
Total number of observations | 246245 | ||
Number of reflections | 49942 | 2408 | 2473 |
<I/σ(I)> | 9.5 | ||
Completeness [%] | 98.2 | 87.2 | 97.9 |
Redundancy | 4.9 | 3.6 | 4.7 |
CC(1/2) | 0.997 | 0.779 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5 | 298 | 0.1 M sodium acetate, pH 5.0, 15% MPD |