6LQE

Crystal structure of Arabidopsis ARID5 PHD finger in complex with H3K4me3 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-25
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9792
Spacegroup name	P 41 21 2
Unit cell lengths	27.922, 27.922, 124.513
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.128 - 1.900
R-factor	0.2026
Rwork	0.201
R-free	0.24090
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.107
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.930
High resolution limit [Å]	1.900	5.160	1.900
Rmerge	0.106	0.078	1.005
Rmeas	0.108	0.080	1.030
Rpim	0.023	0.019	0.220
Number of reflections	4421	289	206
<I/σ(I)>	5.7
Completeness [%]	99.8	99	100
Redundancy	22.3	17.8	21.5
CC(1/2)		0.998	0.883

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	298	0.1 M sodium acetate, pH 4.6, 30% PEG 300