6LPH
the Sufu-Fu complex crystal structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-06-12 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.91878 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 36.145, 68.423, 56.144 |
Unit cell angles | 90.00, 97.49, 90.00 |
Refinement procedure
Resolution | 55.670 - 1.910 |
R-factor | 0.1513 |
Rwork | 0.148 |
R-free | 0.21980 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4kmd |
RMSD bond length | 0.013 |
RMSD bond angle | 1.597 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.670 | 1.980 |
High resolution limit [Å] | 1.910 | 1.910 |
Rmerge | 0.090 | 0.639 |
Number of reflections | 20919 | 20919 |
<I/σ(I)> | 22.3 | |
Completeness [%] | 99.7 | |
Redundancy | 7.5 | |
CC(1/2) | 0.893 | 0.893 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 281 | 1% w/v Tryptone, 0.05M HEPES sodium pH7.0, 20% w/v Polyethylene glycol 3350 |