6LPG

human VASH1-SVBP complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-10
Detector	DECTRIS EIGER2 X 4M
Wavelength(s)	1.1
Spacegroup name	P 61 2 2
Unit cell lengths	71.854, 71.854, 215.811
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.060 - 2.300
R-factor	0.1942
Rwork	0.190
R-free	0.23620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6nvq
RMSD bond length	0.007
RMSD bond angle	0.851
Data reduction software	XDS
Data scaling software	pointless
Phasing software	PHASER
Refinement software	PHENIX (1.17_3644)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.060	2.382
High resolution limit [Å]	2.300	2.300
Rmerge	0.016	0.243
Rmeas	0.023	0.344
Rpim	0.016	0.243
Number of reflections	15488	1492
<I/σ(I)>	24.05	2.59
Completeness [%]	99.9
Redundancy	2
CC(1/2)	1.000	0.860

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.16 M ammonium sulfate, 0.08 M Sodium acetate pH4.6, 20%(w/v) PEG4000, 20%(v/v) glycerol