6LP3

Structural basis and functional analysis epo1-bem3p complex for bud growth

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2018-06-12
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9789
Spacegroup name	P 41 21 2
Unit cell lengths	119.405, 119.405, 144.575
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.032 - 3.547
R-factor	0.3427
Rwork	0.340
R-free	0.39800
Structure solution method	MAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AutoSol
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.032	4.230
High resolution limit [Å]	3.547	3.950
Rmerge	0.431	0.961
Number of reflections	13161	1249
<I/σ(I)>	10.31
Completeness [%]	99.9
Redundancy	18

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	289	1.6 M Ammonium sulfate, 0.1 M MES, pH 6.5, 10% 1,4-Dioxane