6LP3
Structural basis and functional analysis epo1-bem3p complex for bud growth
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-06-12 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9789 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 119.405, 119.405, 144.575 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.032 - 3.547 |
R-factor | 0.3427 |
Rwork | 0.340 |
R-free | 0.39800 |
Structure solution method | MAD |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AutoSol |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.032 | 4.230 |
High resolution limit [Å] | 3.547 | 3.950 |
Rmerge | 0.431 | 0.961 |
Number of reflections | 13161 | 1249 |
<I/σ(I)> | 10.31 | |
Completeness [%] | 99.9 | |
Redundancy | 18 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 1.6 M Ammonium sulfate, 0.1 M MES, pH 6.5, 10% 1,4-Dioxane |