6LP2

Structure of Lpg2148/UBE2N-Ub complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	139.953, 71.266, 75.111
Unit cell angles	90.00, 90.74, 90.00

Refinement procedure

Resolution	31.872 - 2.479
R-factor	0.1781
Rwork	0.175
R-free	0.23260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5suj 1ubq 1j7d
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.568
High resolution limit [Å]	2.479	2.479
Number of reflections	25834	2132
<I/σ(I)>	21.4	4.7
Completeness [%]	99.8
Redundancy	6.2
CC(1/2)	0.987	0.890

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	PEG 3350, potassium citrate