6LOU
Crystal structure of DUSP22 mutant_C88S/S93N
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-11-06 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 90.049, 50.226, 40.938 |
Unit cell angles | 90.00, 93.55, 90.00 |
Refinement procedure
Resolution | 22.471 - 1.530 |
R-factor | 0.1735 |
Rwork | 0.172 |
R-free | 0.19570 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1wrm |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER (2.8.3) |
Refinement software | PHENIX (1.16-3549) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 1.580 |
High resolution limit [Å] | 1.530 | 3.290 | 1.530 |
Rmerge | 0.037 | 0.022 | 0.247 |
Rmeas | 0.043 | 0.025 | 0.312 |
Rpim | 0.023 | 0.013 | 0.187 |
Total number of observations | 92975 | ||
Number of reflections | 27127 | 2810 | 2479 |
<I/σ(I)> | 26.8 | ||
Completeness [%] | 98.3 | 99.1 | 91 |
Redundancy | 3.4 | 3.5 | 2.5 |
CC(1/2) | 0.999 | 0.923 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.05 M arginine, 0.05 M glutamic acid, 0.4 M NaH2PO4, 1.6 M K2HPO4, 0.2 M NaCl |