6LOT
Crystal structure of DUSP22 mutant_N128D
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE TPS 05A |
Synchrotron site | NSRRC |
Beamline | TPS 05A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-11-08 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.99984 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 90.278, 50.686, 41.097 |
Unit cell angles | 90.00, 91.33, 90.00 |
Refinement procedure
Resolution | 25.874 - 1.690 |
R-factor | 0.1946 |
Rwork | 0.192 |
R-free | 0.21920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1wrm |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER (2.8.3) |
Refinement software | PHENIX (1.16-3549) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 1.750 |
High resolution limit [Å] | 1.690 | 3.640 | 1.690 |
Rmerge | 0.045 | 0.021 | 0.350 |
Rmeas | 0.054 | 0.025 | 0.416 |
Rpim | 0.028 | 0.013 | 0.222 |
Total number of observations | 72939 | ||
Number of reflections | 20649 | 2099 | 1926 |
<I/σ(I)> | 16.6 | ||
Completeness [%] | 98.4 | 97.3 | 92.4 |
Redundancy | 3.5 | 3.4 | 3.3 |
CC(1/2) | 0.999 | 0.899 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 293 | 0.1 M PIPES, 30% PEG 3,350, 0.2 M Li2SO4 |