6LOS
Crystal structure of mouse PEDF in complex with heterotrimeric collagen model peptide.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-10-22 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 1.0000 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 95.378, 147.773, 91.548 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.689 - 2.476 |
R-factor | 0.2282 |
Rwork | 0.223 |
R-free | 0.28210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1imv |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.510 |
High resolution limit [Å] | 2.470 | 6.700 | 2.470 |
Rmerge | 0.115 | 0.044 | 0.857 |
Rmeas | 0.124 | 0.048 | 0.934 |
Rpim | 0.046 | 0.018 | 0.366 |
Total number of observations | 157108 | ||
Number of reflections | 22356 | 1268 | 876 |
<I/σ(I)> | 6.7 | ||
Completeness [%] | 95.6 | 99.8 | 75.3 |
Redundancy | 7 | 6.7 | 5.7 |
CC(1/2) | 0.998 | 0.883 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 90 mM CAPS pH 10.5, 1080 mM Sodium phosphate, 720 mM Potassium phosphate and 180 mM Lithium sulfate. |