6LOH
Crystal structure of the catalytic domain of human ubiquitin ligase AREL1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-12-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 150.231, 150.231, 342.645 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.223 - 3.207 |
| Rwork | 0.211 |
| R-free | 0.22490 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.625 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.310 |
| High resolution limit [Å] | 3.207 | 3.250 |
| Rmerge | 0.119 | 0.628 |
| Rmeas | 0.141 | 0.742 |
| Rpim | 0.071 | 0.381 |
| Number of reflections | 38276 | 1756 |
| <I/σ(I)> | 9.6 | 2.06 |
| Completeness [%] | 98.0 | 98.9 |
| Redundancy | 3.4 | 3.5 |
| CC(1/2) | 0.979 | 0.635 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.4 | 289 | 0.1M Sodium phosphate pH 6.4, 0.2M Potassium chloride, 2.25M Sodium chloride |
