6LO9
Crystal structure of RORgammat with ligand C46D bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-11-07 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.987 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 61.583, 61.583, 155.507 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.760 - 1.860 |
R-factor | 0.194340809936 |
Rwork | 0.193 |
R-free | 0.22688 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xyz |
RMSD bond length | 0.007 |
RMSD bond angle | 1.280 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.760 | 1.927 |
High resolution limit [Å] | 1.860 | 1.860 |
Rmerge | 0.934 | |
Rmeas | 0.963 | |
Rpim | 0.233 | |
Number of reflections | 26005 | 2545 |
<I/σ(I)> | 3.26 | 3 |
Completeness [%] | 100.0 | 100 |
Redundancy | 3.26 | |
CC(1/2) | 0.995 | 0.999 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 8% PEG4000, 0.4M NaCl, 0.1M PIPES, pH6.5 |