6LNP

Crystal structure of citrate Biosensor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-30
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.98
Spacegroup name	P 41
Unit cell lengths	92.410, 92.410, 123.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.205 - 2.993
R-factor	0.2287
Rwork	0.223
R-free	0.28230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6gel
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.205	3.100
High resolution limit [Å]	2.990	2.990
Rmerge	0.134
Rmeas	0.143
Number of reflections	20881	2072
<I/σ(I)>	12.1
Completeness [%]	99.8
Redundancy	13.6
CC(1/2)	0.998	0.650

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	4% Tacsimate pH 5.0, 12% Polyethylene glycol 3350