6LNM
Crystal structure of CASK-CaMK in complex with Mint1-CID
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-23 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 31 |
| Unit cell lengths | 151.417, 151.417, 49.455 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.400 - 2.400 |
| R-factor | 0.1734 |
| Rwork | 0.171 |
| R-free | 0.21290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3tac |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.612 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 6.510 | 2.400 |
| Rmerge | 0.167 | 0.056 | 0.992 |
| Rmeas | 0.178 | 0.059 | 1.057 |
| Rpim | 0.058 | 0.018 | 0.358 |
| Total number of observations | 454664 | ||
| Number of reflections | 49568 | 2495 | 2468 |
| <I/σ(I)> | 3.4 | ||
| Completeness [%] | 99.5 | 99.4 | 99.3 |
| Redundancy | 9.2 | 10 | 8.6 |
| CC(1/2) | 0.998 | 0.546 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 6 | 289 | 0.1M BisTris (pH 6.0), 20% PEG 1500 |
