6LN3
Crystal structure of adenylate kinase from an extremophilic archaeon Aeropyrum pernix with ATP and AMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-23 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 193.440, 193.440, 193.440 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.700 - 2.000 |
| R-factor | 0.1612 |
| Rwork | 0.160 |
| R-free | 0.19180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kht |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.959 |
| Data reduction software | HKL-2000 (1.98.5) |
| Data scaling software | HKL-2000 (1.98.5) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.068 | 0.306 |
| Number of reflections | 20843 | 1679 |
| <I/σ(I)> | 79.6 | 11.4 |
| Completeness [%] | 96.9 | 80.5 |
| Redundancy | 37.8 | 25.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.6 | 293 | 0.2 M Ammonium sulfate, 0.1 M HEPES pH 7.6, 25% (w/v) Polyethylene glycol monomethyl ether 2000, 5 mM adenosine 5'-triphosphate, 5 mM magnesium ions |
