6LMJ

ASFV pA104R in complex with double-strand DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-25
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.98
Spacegroup name	C 1 2 1
Unit cell lengths	82.358, 89.095, 53.227
Unit cell angles	90.00, 100.16, 90.00

Refinement procedure

Resolution	27.886 - 2.800
R-factor	0.2572
Rwork	0.256
R-free	0.28480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6lmh
RMSD bond length	0.004
RMSD bond angle	0.703
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.15.2_3472: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.800	2.800
Number of reflections	9113	931
<I/σ(I)>	20.619
Completeness [%]	96.2
Redundancy	6.4
CC(1/2)	1.000	0.790

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	0.2 M imidazole malate, pH 7.0, 15% (w/v) PEG 4000