6LL6

Crsyal structure of EcFtsZ (residues 11-316)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-23
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0
Spacegroup name	P 1
Unit cell lengths	38.280, 41.670, 45.030
Unit cell angles	88.79, 72.06, 72.44

Refinement procedure

Resolution	42.710 - 2.500
R-factor	0.1865
Rwork	0.184
R-free	0.24150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	unreleased
RMSD bond length	0.008
RMSD bond angle	1.008
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.560
High resolution limit [Å]	2.500	2.500
Number of reflections	8681	869
<I/σ(I)>	5.64
Completeness [%]	99.6
Redundancy	6.3
CC(1/2)	0.975	0.856

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PCB buffer, PEG 1500