6LL3

Crystal structure of lysozyme by fixed-target serial femtosecond crystallography

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	FREE ELECTRON LASER
Source details	PAL-XFEL BEAMLINE NCI
Synchrotron site	PAL-XFEL
Beamline	NCI
Temperature [K]	297
Detector technology	CCD
Collection date	2019-11-09
Detector	RAYONIX MX225-HS
Wavelength(s)	1.2782
Spacegroup name	P 43 21 2
Unit cell lengths	78.980, 78.980, 38.220
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.847 - 1.850
R-factor	0.207
Rwork	0.203
R-free	0.24620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ig6
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	MOLREP
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	72.400	1.860
High resolution limit [Å]	1.800	1.800
Number of reflections	11728	1138
<I/σ(I)>	6.66	2.06
Completeness [%]	100.0
Redundancy	155.1
CC(1/2)	0.975	0.712

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES	4.5	293.5	sodium acetate, PEG 8000, NaCl