6LKR

Crystal structure of mouse DCAR2 CRD domain complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45XU
Synchrotron site	SPring-8
Beamline	BL45XU
Temperature [K]	100
Detector technology	CCD
Collection date	2019-11-22
Detector	ADSC QUANTUM 315
Wavelength(s)	1.00000
Spacegroup name	P 21 21 21
Unit cell lengths	64.423, 72.068, 100.580
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.030 - 1.840
R-factor	0.1726
Rwork	0.172
R-free	0.18840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6lfj
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.030	1.950
High resolution limit [Å]	1.840	1.840
Number of reflections	77390	12362
<I/σ(I)>	9.8
Completeness [%]	99.3
Redundancy	3.5
CC(1/2)	0.998	0.519

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.4	PEG