6LKP

Crystal structure of Dps1 from the thermophilic non-heterocystous filamentous cyanobacterium Thermoleptolyngbya sp. O-77

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45XU
Synchrotron site	SPring-8
Beamline	BL45XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.25
Spacegroup name	P 43 2 2
Unit cell lengths	92.787, 92.787, 261.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.390 - 2.900
R-factor	0.2574
Rwork	0.253
R-free	0.31490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5hjh
RMSD bond length	0.005
RMSD bond angle	0.812
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.390	3.000
High resolution limit [Å]	2.900	2.900
Rmerge	0.128	1.606
Rmeas	0.133	1.669
Rpim	0.035	0.455
Number of reflections	48249	4857
<I/σ(I)>	12.1	1.2
Completeness [%]	100.0	100
Redundancy	14	13.3
CC(1/2)	1.000	0.627

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.15 M Cesium chloride and 18% Polyethylene glycol 3350