6LKP
Crystal structure of Dps1 from the thermophilic non-heterocystous filamentous cyanobacterium Thermoleptolyngbya sp. O-77
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL45XU |
Synchrotron site | SPring-8 |
Beamline | BL45XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-12-02 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.25 |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 92.787, 92.787, 261.120 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.390 - 2.900 |
R-factor | 0.2574 |
Rwork | 0.253 |
R-free | 0.31490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5hjh |
RMSD bond length | 0.005 |
RMSD bond angle | 0.812 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.390 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.128 | 1.606 |
Rmeas | 0.133 | 1.669 |
Rpim | 0.035 | 0.455 |
Number of reflections | 48249 | 4857 |
<I/σ(I)> | 12.1 | 1.2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 14 | 13.3 |
CC(1/2) | 1.000 | 0.627 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.15 M Cesium chloride and 18% Polyethylene glycol 3350 |