6LKJ
Two-component system protein mediate signal transduction
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-09-05 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9793 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 52.164, 67.157, 87.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.235 - 2.004 |
R-factor | 0.1968 |
Rwork | 0.195 |
R-free | 0.22940 |
Structure solution method | SAD |
RMSD bond length | 0.002 |
RMSD bond angle | 0.525 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | REFMAC |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.110 | 0.483 |
Rmeas | 0.124 | 0.565 |
Rpim | 0.054 | 0.287 |
Number of reflections | 21110 | 2042 |
<I/σ(I)> | 11.05 | 2.82 |
Completeness [%] | 99.3 | 100 |
Redundancy | 4.9 | 3.8 |
CC(1/2) | 0.995 | 0.751 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 0.1M Tris pH 7.5-7.9, 21% PEG 3350 |