6LKG
two-component system protein mediate signal transduction
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-05 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.300, 89.970, 92.827 |
| Unit cell angles | 90.00, 100.87, 90.00 |
Refinement procedure
| Resolution | 38.161 - 1.948 |
| R-factor | 0.2047 |
| Rwork | 0.204 |
| R-free | 0.22450 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.783 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.980 |
| High resolution limit [Å] | 1.948 | 1.950 |
| Rmerge | 0.085 | 0.851 |
| Rmeas | 0.092 | 0.926 |
| Rpim | 0.035 | 0.361 |
| Number of reflections | 85134 | 8279 |
| <I/σ(I)> | 22.0625 | 1.875 |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 6.8 | 6.4 |
| CC(1/2) | 0.994 | 0.772 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 293 | 0.1M HEPES pH 7.0, 18% PEG 4000 |
