6LK9
Coho salmon ferritin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 93 |
Detector technology | PIXEL |
Collection date | 2019-01-13 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9785 |
Spacegroup name | F 4 3 2 |
Unit cell lengths | 182.092, 182.092, 182.092 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.775 - 2.099 |
R-factor | 0.1978 |
Rwork | 0.196 |
R-free | 0.23280 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fha |
RMSD bond length | 0.006 |
RMSD bond angle | 0.781 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.775 | 2.174 |
High resolution limit [Å] | 2.099 | 2.099 |
Number of reflections | 15705 | 1535 |
<I/σ(I)> | 1.35 | |
Completeness [%] | 100.0 | |
Redundancy | 1 | |
CC(1/2) | 1.000 | 1.000 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | PEG 2000 MME,Tris base |