6LK3
The Functional Characterization and Crystal Structure of Type II Peptidyl Carrier Protein ColA1a in Collismycins Biosynthesis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-13 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97917 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.697, 79.027, 34.088 |
| Unit cell angles | 90.00, 111.29, 90.00 |
Refinement procedure
| Resolution | 39.510 - 2.100 |
| R-factor | 0.1519 |
| Rwork | 0.149 |
| R-free | 0.20210 |
| Structure solution method | SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.900 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.510 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.139 | 0.473 |
| Number of reflections | 9816 | 503 |
| <I/σ(I)> | 4.6 | |
| Completeness [%] | 98.1 | |
| Redundancy | 13.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.2 M sodium chloride, 0.1 M Bis-Tris pH 6.25, 25% w/v polyethylene glycol 3,350 |
