6LK1
Ultrahigh resolution X-ray structure of Ferredoxin I from C. reinhardtii
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-01-17 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.9 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 25.955, 60.090, 50.085 |
Unit cell angles | 90.00, 102.40, 90.00 |
Refinement procedure
Resolution | 37.960 - 0.900 |
Rwork | 0.145 |
R-free | 0.17130 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1awd |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | SHELX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.960 | 0.920 |
High resolution limit [Å] | 0.900 | 0.900 |
Rmerge | 0.061 | 0.729 |
Rmeas | 0.350 | |
Number of reflections | 109765 | 7995 |
<I/σ(I)> | 12.9 | 1.7 |
Completeness [%] | 99.2 | 97.6 |
Redundancy | 4.736 | 3.188 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 298 | Ammonium sulfate Sodium citrate Sodium thiocyanate Benzamidine Hydrochloride |