6LK1
Ultrahigh resolution X-ray structure of Ferredoxin I from C. reinhardtii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-01-17 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 25.955, 60.090, 50.085 |
| Unit cell angles | 90.00, 102.40, 90.00 |
Refinement procedure
| Resolution | 37.960 - 0.900 |
| Rwork | 0.145 |
| R-free | 0.17130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1awd |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | SHELX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.960 | 0.920 |
| High resolution limit [Å] | 0.900 | 0.900 |
| Rmerge | 0.061 | 0.729 |
| Rmeas | 0.350 | |
| Number of reflections | 109765 | 7995 |
| <I/σ(I)> | 12.9 | 1.7 |
| Completeness [%] | 99.2 | 97.6 |
| Redundancy | 4.736 | 3.188 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 298 | Ammonium sulfate Sodium citrate Sodium thiocyanate Benzamidine Hydrochloride |
