6LJR
human galectin-16 R55N/H57R
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-10-31 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.98 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 55.923, 34.405, 67.995 |
Unit cell angles | 90.00, 102.25, 90.00 |
Refinement procedure
Resolution | 19.330 - 2.000 |
R-factor | 0.1666 |
Rwork | 0.161 |
R-free | 0.21670 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6ljp |
Refinement software | PHENIX (1.17_3644) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.330 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.039 | 0.124 |
Number of reflections | 8674 | 647 |
<I/σ(I)> | 16 | |
Completeness [%] | 99.4 | |
Redundancy | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | PEG |