6LJ9
Crystal Structure of Se-Met ASFV pS273R protease
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-04-17 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97911 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 60.165, 83.280, 108.724 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.769 - 2.307 |
R-factor | 0.1929 |
Rwork | 0.191 |
R-free | 0.22020 |
Structure solution method | SAD |
Data reduction software | XDS |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.843 | 2.200 |
High resolution limit [Å] | 2.079 | 2.079 |
Number of reflections | 21872 | 19824 |
<I/σ(I)> | 10.67 | |
Completeness [%] | 100.0 | |
Redundancy | 7.04 | |
CC(1/2) | 0.999 | 0.715 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 289 | 0.2 M Lithium sulfate monohydrate, 0.1 M Tris, pH 8.2, 23% w/v Polyethylene glycol 1,500 |