6LIY
SeMet CRL Protein of Arabidopsis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-06-05 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9791 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 38.289, 73.540, 67.147 |
Unit cell angles | 90.00, 97.16, 90.00 |
Refinement procedure
Resolution | 32.193 - 1.761 |
R-factor | 0.2088 |
Rwork | 0.207 |
R-free | 0.24390 |
Structure solution method | SAD |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.790 |
High resolution limit [Å] | 1.760 | 4.780 | 1.760 |
Rmerge | 0.082 | 0.066 | 0.825 |
Rmeas | 0.089 | 0.072 | 0.888 |
Rpim | 0.033 | 0.027 | 0.324 |
Total number of observations | 267556 | ||
Number of reflections | 35639 | 1808 | 1753 |
<I/σ(I)> | 13.4 | ||
Completeness [%] | 97.2 | 95.9 | 96 |
Redundancy | 7.5 | 6.8 | 7.4 |
CC(1/2) | 0.996 | 0.845 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 277 | 0.1 M MES pH 6.5, 12% PEG 20000 |