6LIY

SeMet CRL Protein of Arabidopsis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-05
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9791
Spacegroup name	P 1 21 1
Unit cell lengths	38.289, 73.540, 67.147
Unit cell angles	90.00, 97.16, 90.00

Refinement procedure

Resolution	32.193 - 1.761
R-factor	0.2088
Rwork	0.207
R-free	0.24390
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.790
High resolution limit [Å]	1.760	4.780	1.760
Rmerge	0.082	0.066	0.825
Rmeas	0.089	0.072	0.888
Rpim	0.033	0.027	0.324
Total number of observations	267556
Number of reflections	35639	1808	1753
<I/σ(I)>	13.4
Completeness [%]	97.2	95.9	96
Redundancy	7.5	6.8	7.4
CC(1/2)		0.996	0.845

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	277	0.1 M MES pH 6.5, 12% PEG 20000