6LIK
Crystal Structure of Lectin from Pleurotus ostreatus in complex with Galactose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-03-01 |
Detector | DECTRIS EIGER2 XE 16M |
Wavelength(s) | 0.99 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 152.507, 152.507, 146.062 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.040 - 2.400 |
R-factor | 0.1862 |
Rwork | 0.185 |
R-free | 0.21290 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6kbq |
RMSD bond length | 0.014 |
RMSD bond angle | 2.041 |
Data reduction software | HKL-2000 |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.040 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmeas | 0.146 | |
Rpim | 0.043 | 0.423 |
Number of reflections | 39208 | 3803 |
<I/σ(I)> | 12.98 | 1.58 |
Completeness [%] | 99.0 | |
Redundancy | 5.1 | |
CC(1/2) | 0.994 | 0.604 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 300 | 0.9M Na2HPO4/K2HPO4, pH 5.5, 33% glycerol |