6LI7

Crystal Structure of Lectin from Pleurotus ostreatus in complex with GalNAc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-02
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.99
Spacegroup name	P 61 2 2
Unit cell lengths	152.273, 152.273, 145.975
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.642 - 2.098
Rwork	0.193
R-free	0.20550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3w5n
RMSD bond length	0.016
RMSD bond angle	2.098
Data reduction software	HKL-2000
Data scaling software	SCALA
Phasing software	BALBES
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.180
High resolution limit [Å]	2.098	2.100
Number of reflections	58637	5767
<I/σ(I)>	16.3
Completeness [%]	99.9
Redundancy	5
CC(1/2)	0.996	0.996

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	300	0.9M Na2HPO4/K2HPO4, pH 5.5, 33% glycerol