6LHZ
Structure of aerolysin-like protein (Bombina maxima)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-03-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 79.082, 79.082, 48.687 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.720 - 2.520 |
| R-factor | 0.1969 |
| Rwork | 0.193 |
| R-free | 0.26450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6lh8 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.560 |
| High resolution limit [Å] | 2.520 | 2.520 |
| Rmerge | 0.117 | 0.492 |
| Number of reflections | 5562 | 3919 |
| <I/σ(I)> | 18.5 | 12.571 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 13.6 | 14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 289 | 0.2 M magnesium chloride hexahydrate, 25% polyethylene glycol 3350, 0.1 M Bis-tris |
