6LHR
Crystal structure of the complex between Vesicle Amine Transport-1 and NADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-12 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 176.258, 96.290, 112.350 |
| Unit cell angles | 90.00, 123.13, 90.00 |
Refinement procedure
| Resolution | 48.150 - 2.620 |
| R-factor | 0.2095 |
| Rwork | 0.207 |
| R-free | 0.24620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4a27 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.955 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.770 |
| High resolution limit [Å] | 2.620 | 2.620 |
| Rmerge | 0.095 | 0.640 |
| Number of reflections | 46822 | 7533 |
| <I/σ(I)> | 10.02 | |
| Completeness [%] | 98.5 | |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG 3000, Sodium citrate |
