6LHH

Crystal structure of chicken 8mer-BF2*1501

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-08-12
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97
Spacegroup name	P 31 2 1
Unit cell lengths	123.452, 123.452, 81.220
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.710
R-factor	0.2321
Rwork	0.230
R-free	0.26470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4e0r
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.770
High resolution limit [Å]	2.710	2.710
Rmerge	0.080	0.240
Number of reflections	18044	111237
<I/σ(I)>	11.09	2.659
Completeness [%]	96.2
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.8	291	0.2M Magnesium nitrate hexahydrate, 20% (w/v) polyethylene glycol 3350, PH 5.8