6LHG

Crystal structure of chicken cCD8aa/pBF2*04:01

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-12-10
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97
Spacegroup name	P 1 21 1
Unit cell lengths	90.576, 90.817, 94.874
Unit cell angles	90.00, 98.61, 90.00

Refinement procedure

Resolution	29.869 - 2.800
Rwork	0.252
R-free	0.28810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4e0r 1cd8
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.790	2.800
Rmerge	0.105	0.613
Number of reflections	37579	163307
<I/σ(I)>	14.14
Completeness [%]	99.8
Redundancy	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	0.2 M ammonium sulfate, 0.1 M Bis-tris pH 6.5, 25% (w/v) polyethylene glycol 3350