6LHF
Crystal structure of chicken cCD8aa/pBF2*15:01
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-03-12 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97972 |
Spacegroup name | P 1 |
Unit cell lengths | 51.340, 66.735, 103.949 |
Unit cell angles | 84.59, 82.04, 67.51 |
Refinement procedure
Resolution | 29.893 - 2.700 |
Rwork | 0.253 |
R-free | 0.29900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4e0r |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.109 | 0.516 |
Number of reflections | 36586 | 108500 |
<I/σ(I)> | 11.587 | |
Completeness [%] | 95.8 | 98 |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M sodium chloride, 25% (w/v) polyethylene glycol 3,350,0.1 M HEPES pH 7.5 |