6LH8
Structure of aerolysin-like protein (Bombina maxima)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-07-14 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9793 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 78.793, 78.793, 48.948 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.578 - 1.729 |
R-factor | 0.196 |
Rwork | 0.192 |
R-free | 0.23110 |
Structure solution method | SAD |
Data scaling software | SCALEPACK (HKL2000) |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.580 | 1.760 |
High resolution limit [Å] | 1.729 | 1.729 |
Number of reflections | 16266 | 1522 |
<I/σ(I)> | 41.88 | 2.62 |
Completeness [%] | 100.0 | 100 |
Redundancy | 13.3 | |
CC(1/2) | 0.999 | 0.884 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 289 | 0.2M magnesium chloride hexahydrate, 25% PEG 3350, 0.1M bis-tris |