6LGO
Crystal structure of cystathionine gamma synthase from Xanthomonas oryzae pv. oryzae in complex with homolanthionine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 41 |
| Unit cell lengths | 161.222, 161.222, 244.914 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.070 - 2.390 |
| R-factor | 0.1551 |
| Rwork | 0.154 |
| R-free | 0.18130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ld7 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.427 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.480 |
| High resolution limit [Å] | 2.390 | 2.390 |
| Rmerge | 0.083 | 0.260 |
| Number of reflections | 117802 | 8983 |
| <I/σ(I)> | 8.1 | |
| Completeness [%] | 96.2 | |
| Redundancy | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 287 | 0.12M Ethylene Glycols Mix, 0.1M Na HEPES/MOPS pH 7.5, 10%(w/v) PEG 4000, 20%(v/v) glycerol |
