6LGO

Crystal structure of cystathionine gamma synthase from Xanthomonas oryzae pv. oryzae in complex with homolanthionine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2013-04-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	I 41
Unit cell lengths	161.222, 161.222, 244.914
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.070 - 2.390
R-factor	0.1551
Rwork	0.154
R-free	0.18130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ld7
RMSD bond length	0.019
RMSD bond angle	2.427
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.480
High resolution limit [Å]	2.390	2.390
Rmerge	0.083	0.260
Number of reflections	117802	8983
<I/σ(I)>	8.1
Completeness [%]	96.2
Redundancy	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	287	0.12M Ethylene Glycols Mix, 0.1M Na HEPES/MOPS pH 7.5, 10%(w/v) PEG 4000, 20%(v/v) glycerol