6LGH
Bombyx mori GH13 sucrose hydrolase mutant E322Q covalent intermediate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-23 |
| Detector | DECTRIS PILATUS3 S 2M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.360, 128.300, 154.420 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.490 - 1.700 |
| R-factor | 0.1845 |
| Rwork | 0.183 |
| R-free | 0.21120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5brq |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.345 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.127 | 0.683 |
| Number of reflections | 140237 | 20187 |
| <I/σ(I)> | 7.9 | 2 |
| Completeness [%] | 99.5 | 99.1 |
| Redundancy | 4.7 | 4.3 |
| CC(1/2) | 0.989 | 0.672 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 10% PEG 3350, 0.2 M magnesium acetate, 10 mM alpha-glucopyranosyl fluoride |
