6LGG
Bombyx mori GH13 sucrose hydrolase mutant E322Q complexed with sucrose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-11-03 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 64.700, 127.900, 154.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.280 - 1.840 |
R-factor | 0.1823 |
Rwork | 0.181 |
R-free | 0.21140 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5brq |
RMSD bond length | 0.010 |
RMSD bond angle | 1.600 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.940 |
High resolution limit [Å] | 1.840 | 1.840 |
Rmerge | 0.097 | 0.775 |
Number of reflections | 111648 | 16116 |
<I/σ(I)> | 12.4 | 2.4 |
Completeness [%] | 99.9 | 100 |
Redundancy | 6.5 | 6.7 |
CC(1/2) | 0.998 | 0.769 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 8% PEG 3350, 0.2 M magnesium acetate, 10 mM sucrose |