6LGD
Bombyx mori GH13 sucrose hydrolase complexed with 1,4-dideoxy-1,4-imino-D-arabinitol
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-11-24 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 65.054, 146.444, 153.291 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.290 - 1.750 |
R-factor | 0.1632 |
Rwork | 0.162 |
R-free | 0.18370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5brq |
RMSD bond length | 0.005 |
RMSD bond angle | 1.301 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.840 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.074 | 0.889 |
Number of reflections | 148203 | 21397 |
<I/σ(I)> | 15.4 | 2.2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 6.6 | 6.7 |
CC(1/2) | 0.999 | 0.755 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 8% PEG 3350, 0.2 M magnesium acetate, 10 mM 1,4-dideoxy-1,4-imino-D-arabinitol |