6LFU
Poa1p F152A mutant in complex with ADP-ribose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-11-02 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.399, 68.509, 55.879 |
Unit cell angles | 90.00, 91.68, 90.00 |
Refinement procedure
Resolution | 24.884 - 3.123 |
R-factor | 0.1954 |
Rwork | 0.188 |
R-free | 0.25780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6lfq |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.884 | 3.436 |
High resolution limit [Å] | 3.123 | 3.123 |
Number of reflections | 5850 | |
<I/σ(I)> | 20.5 | |
Completeness [%] | 98.0 | |
Redundancy | 3.5 | |
CC(1/2) | 0.985 | 0.953 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 283 | Citric acid, PEG 3350, Glycerol |